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Hari, Sowmya
- Screening of Enzymes from Actinomycetes and Fungi isolated from Plastic Dumped Soil
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Authors
Affiliations
1 Department of Bio-Engineering, School of Engineering, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-600117, Tamilnadu, IN
1 Department of Bio-Engineering, School of Engineering, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-600117, Tamilnadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 5 (2019), Pagination: 2261-2266Abstract
Enzymes as catalysts play a major role in day to day lives by catalyzing major biochemical and metabolic interconversions. Microbes are chief source of enzymes and synthesize them for the production of secondary metabolites. These enzymes can be exploited to develop many novel functions. In this study, actinomycetes and fungi were isolated from plastic waste dumped soil. A total of 35 actinomycetes and 15 fungal species were isolated from first level screening. Among them 5 actinomycetes and 3 fungal strains that showed better growth were chosen for further enzyme screening and characterization studies. These strains were screened for four enzymes viz. cellulase, protease, xylanase and glucosidase production. Actinomycetes showed xylanase and cellulase producing ability. The fungal strain in addition to the production of xylanase, protease enzymes also showed cellulase production. One actinomycetes and fungal strain that showed multienzyme production were subjected to further biochemical and morphological characterization. From morphological observation, actinomycetes revealed a creamy white, long spored species with some producing a light yellow pigmentation. The fungal strains showed black spores at the centre with surrounding white filaments. The fungal strain was also confirmed by visualizing spores and hyphae under the microscope. The actinomycetes were subjected to various biochemical tests. The tests revealed the strain isolated was an actinomycete. In future, these fungi and actinomycete can be exploited to produce the enzymes in large scale which can be screened for their ability to degrade plastic.Keywords
Soil, Plastic, Actinomycetes, Fungi, Protease, Cellulase, Glucosidase, Xylanase.
References
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- In Silico Homology Modeling of Presenilin 2- Therapeutic Target for Alzheimer’s Disease
Abstract Views :396 |
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Authors
Sowmya Hari
1,
S. Akilashree
1
Affiliations
1 Department of Bio-Engineering, School of Engineering, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-600117, Tamilnadu, IN
1 Department of Bio-Engineering, School of Engineering, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-600117, Tamilnadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 7 (2019), Pagination: 3405-3409Abstract
Alzheimer’s disease is a neurodegenerative disorder which is caused by several mutations in causative proteins like Amyloid Precursor Protein (APP), Presenilin1 (PSEN1), and Presenilin2 (PSEN2). Mutations in presenilin 2 lead to the overproduction of amyloid beta peptide which gets accumulated in the brain and causes neuron death. This result in Early Onset Alzheimer’s disease (EOAD) and there are around 17 mutations in presenilin 2 results in this disease condition. There are 3 isoforms of PSEN2 which are normally found in the cells. Three dimensional structure of presenilin 2 has not been determined; hence, in this study the structure of presenilin 2 was predicted using homology modeling. The 3dimensional structures are modeled using Modeller9.20, Swiss Model and Geno3D. The structures are validated using PROCHECK. The structure of Swiss Model was found to be more reliable with fewer amino acids in the disallowed region, followed by Modeller9.0 and Geno3D. These structures will be fundamental in determining the crystal structure of presenilin 2 and for drug discovery.Keywords
Homology Modeling, Presenilin 2, Alzheimer’s Disease, Modeller9.20, Swiss Model, Geno3D.References
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